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recombinant proteins recombinant human il 13 protein r d systems  (R&D Systems)


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    R&D Systems recombinant proteins recombinant human il 13 protein r d systems
    Recombinant Proteins Recombinant Human Il 13 Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+proteins+recombinant+human+il+13+protein+r+d+systems/Recombinant+Human+IL-13+Protein/pm41446728-554-130-136
    Average 96 stars, based on 222 article reviews
    recombinant proteins recombinant human il 13 protein r d systems - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..

    Virus:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..

    shRNA:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..

    Plasmid Preparation:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..

    Control:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..

    Sequencing:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..

    CCK-8 Assay:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..

    Software:

    Article Title: Single-cell transcriptomic landscape of keratinocyte transformation from actinic keratosis to skin carcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-IL13RA1 antibody Abcam Cat# ab79277; RRID: AB_1658891 Rabbit anti-IL4R antibody Cell Signaling Technology Cat# 8720; RRID: AB_10829029 Rabbit anti-Bcl-xL antibody AiFang biological Cat# AFRM9153; RRID: AB_10695839 Rabbit anti-Cytokeratin 17 antibody AiFang biological Cat# AFRM80512; RRID: AB_881523 Rabbit GAPDH Monoclonal antibody (1E6D9) proteintech Cat# 60004-1-Ig; RRID: AB_2107436 Rabbit Alpha Tubulin Recombinant monoclonal antibody proteintech Cat# 80762-1-RR; RRID: AB_2918911 Mouse anti-β-actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 HRP-conjugated goat anti-rabbit IgG (H + L) proteintech Cat# SA00001-2; RRID: AB_2722564 HRP-conjugated goat anti-mouse IgG(H + L) proteintech Cat# SA00001-1; RRID: AB_2722565 Bacterial and virus strains Lentiviral shRNA plasmid pLKO.1 targeting IL13RA1 Zorin (Shanghai, China) N/A Lentiviral shRNA plasmid pLKO.1 targeting IL4R Zorin (Shanghai, China) N/A Control shRNA (scramble sequence) Zorin (Shanghai, China) N/A Chemicals, peptides, and recombinant proteins Recombinant human IL-13 protein R&D Systems Cat# 213-IL-005 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833 DMEM (high glucose) Gibco Cat# 11965-092 Fetal bovine serum (FBS) Gibco Cat# 10270-106 Penicillin-streptomycin solution Gibco Cat# 15140-122 Trypsin-EDTA (0.25%) Gibco Cat# 25200-056 Critical commercial assays Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 RNAiso Plus Takara Cat# 9109 PrimeScript RT Reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq II Takara Cat# RR820A Deposited data This paper GEO GSE200334 Experimental models: Cell lines HaCaT (human keratinocyte cell line) JCRB Cell Bank Cat# JCRB0168 HSC-5 (human cSCC cell line) JCRB Cell Bank Cat# JCRB0622 Software and algorithms R (v 4.0.2) R Core Team 44 https://cran.r-project.org/bin/ R Studio (v 1.4.1717) R Studio Team 45 https://www.rstudio.com/ Seurat (v4.0) Hao et al. 46 http://satijalab.org/seurat Cell Ranger (v2.1.0) Zheng et al. 47 https://www.10xgenomics.com/support/software/ cell-ranger/latest STAR (v2.7.10a) Dobin et al. 48 https://github.com/alexdobin/STAR Scrublet (v0.2.2) Wolock et al. 49 https://github.com/AllonKleinLab/scrublet Monocle3 Trapnell et al. 50 https://cole-trapnell-lab.github.io/monocle3/ NicheNet (v1.1.0) Browaeys et al. 51 https://github.com/saeyslab/nichenetr SingleR (v1.4.1) Aran et al. 52 https://github.com/dviraran/SingleR (Continued on next page) iScience 28, 114169, December 19, 2025 e1 ..



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